The effect of different lyoprotectants (sucrose, dimethyl-succinate buffer (DMS), bovine serum albumin (BSA), mannitol and dextran, mw 60kDa) on the stability of the enzyme lignin peroxidase (LiP, EC number: 1.11.1.-), both during the freeze-drying process and storage were investigated. The shelf stability tests were performed at 4°C and 27°C. Both DMS buffer and sucrose showed a good protective action: the former was particularly effective during the process, while the latter improved the stability during storage. In contrast, mannitol and dextran had negative effects, reducing the activity also in the lignin peroxidase solution. BSA was discarded because, in the range of compatible concentrations with LiP, it does not confer a consistent structure to the freeze-dried product.

Freeze-drying of lignin peroxidase: influence on enzyme activity and stability / Capolongo, Antonio; Barresi, Antonello; Rovero, Giorgio. - In: JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY. - ISSN 0268-2575. - STAMPA. - 78:1(2003), pp. 56-63. [10.1002/jctb.742]

Freeze-drying of lignin peroxidase: influence on enzyme activity and stability

CAPOLONGO, Antonio;BARRESI, Antonello;ROVERO, Giorgio
2003

Abstract

The effect of different lyoprotectants (sucrose, dimethyl-succinate buffer (DMS), bovine serum albumin (BSA), mannitol and dextran, mw 60kDa) on the stability of the enzyme lignin peroxidase (LiP, EC number: 1.11.1.-), both during the freeze-drying process and storage were investigated. The shelf stability tests were performed at 4°C and 27°C. Both DMS buffer and sucrose showed a good protective action: the former was particularly effective during the process, while the latter improved the stability during storage. In contrast, mannitol and dextran had negative effects, reducing the activity also in the lignin peroxidase solution. BSA was discarded because, in the range of compatible concentrations with LiP, it does not confer a consistent structure to the freeze-dried product.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11583/1667504
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